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Tol2 8xSTAR-mScarletI-NLS. PGK-puro

Plasmid

#136263

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT001

Plasmid

#171061

Purpose

Plasmid for expressing HIV-1 envelope glycoprotein

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

HIV-1 Env

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_TIA1

Plasmid

#106093

Purpose

gene expression of FLAGHA-TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_04_01

Plasmid

#165013

Purpose

Expression of HLA-DRB1*04:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*04:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI. PGK-puro

Plasmid

#136266

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-sTomato. PGK-puro

Plasmid

#136264

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTetO-hOSK-CASH-1

Plasmid

#188977

Purpose

A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

Doxycycline inducible reprogramming donor cassette

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-tagRFP

Plasmid

#185599

Purpose

Lentiviral construct for expression of tagRFP-T targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80L SpyTag-ELP-SpyTag DK

Plasmid

#112635

Purpose

SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag DK

Tags

His6

Expression

Bacterial

Mutation

Contains central cysteine. Reactive aspartic acid…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gβγ-BERKY3

Plasmid

#158219

Purpose

BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Promoter

CMV/R/U5

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21

Plasmid

#83421

Purpose

Mouse Rab21 (wild type)

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Article

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Pprm1-luciferase; his-3

Plasmid

#137728

Purpose

Communication-activated luciferase in Neurospora crassa

Depositor

Louise Glass

Article

Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

Inserts

Luciferase
Pprm-1 Promoter

Use

Luciferase; Neurospora crassa expression

Mutation

Codon-optimized for N. crassa

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSB-BbsI-sgRNA

Plasmid

#203359

Purpose

Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

BbsI Cut Sites

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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