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We narrowed to 25,285 results for: crispr

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Showing: 4721 - 4740 of 25285 results

  1. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid
    #191528
    Purpose
    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain
    Depositor
    Yannick Doyon
    Insert
    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191528-plasmid-map-sequence-id-378116

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  2. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid
    #220143
    Purpose
    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression
    Depositor
    Frank Buchholz
    Publication
    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Zim3-MeCP2
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    Tre-3G
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220143-plasmid-map-sequence-id-435331

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  4. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    183964-plasmid-map-sequence-id-363120

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  5. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171098-plasmid-map-sequence-id-336112

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  6. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Publication
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  7. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Publication
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    6xHis tag, (-30)dGFP, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  8. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    109121-plasmid-map-sequence-id-209767

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  16. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Promoter
    SFFV
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

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  17. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Publication
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    117686-plasmid-map-sequence-id-236350

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Showing: 4721 - 4740 of 25285 results