We narrowed to 246,220 results for: ins

1. pNCST-AausFP4

   Plasmid

   #129502

   Purpose

   Expresses AausFP4 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP4
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. STK16_HUMAN_D0

   Plasmid

   #79701

   Purpose

   This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK16 (STK16 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. ZIKV_NASBA_27B

   Plasmid

   #75010

   Purpose

   Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_NASBA_27B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-TEV-FLAG

   Plasmid

   #105783

   Purpose

   Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX CblC-SH2

   Plasmid

   #46414

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Cblc CBLC (CBLC Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 8-326

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST40-2XHA-Src-S4-UD

   Plasmid

   #140304

   Purpose

   Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST40-2XHA-Src-S4-KD

   Plasmid

   #140296

   Purpose

   Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and KD regions

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XHA-Src-S4-UD-KD

   Plasmid

   #140300

   Purpose

   Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-ZZ-TEV-BICDR1

    Plasmid

    #111859

    Purpose

    Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, TEV site, and ZZ

    Expression

    Insect

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX Grb7-SH2

    Plasmid

    #46444

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb7 (GRB7 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 417-532

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Blk-SH2

    Plasmid

    #46407

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Blk (BLK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 119-224

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX p85b(N)-SH2

    Plasmid

    #46467

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85b(N) (PIK3R2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 318-431

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1α promoter

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p13xLexAOP2-IVS-Syn21-Voltron-p10

    Plasmid

    #119042

    Purpose

    LexAop2-driven expression of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX p85b(C)-SH2

    Plasmid

    #46466

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85b(C) (PIK3R2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 610-728

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. RAMA-bio

    Plasmid

    #47786

    Purpose

    Expresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAMA
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Socs4-SH2

    Plasmid

    #46507

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs4 (SOCS6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 376-498

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX tensin-SH2

    Plasmid

    #46525

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    tensin (TNS1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1451-1588

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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