We narrowed to 11,558 results for: kars;
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Intron-Tagging-mScarlet-Frame1-Parental-Minicircle
Plasmid#216125PurposeParental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.DepositorInsertsgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
UseCRISPRAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hSox2
Plasmid#20073DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
ZFP36L2
Plasmid#156149PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-C7
Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(NC)-SH2
Plasmid#46496DepositorInsertSHP-1(NC) (PTPN6 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-221PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD8-mCherry-FRB
Plasmid#100738PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Cis1-SH2
Plasmid#46417DepositorInsertCis1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 90-214PromoterTacAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs3-SH2
Plasmid#46506DepositorInsertSocs3 (SOCS3 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 22-142PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hKlf4
Plasmid#20074DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Junb
Plasmid#178441Purposedoxycycline-inducible expression of Mouse Junb in mammalian cellsDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(NC)-SH2
Plasmid#46535DepositorInsertZap70(NC) (ZAP70 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-268PromoterTacAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorInsertESR1-enhancer (ESR1 Human)
UseLuciferaseAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-epsin(144-575)-GFP
Plasmid#100733PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertepn1 (Epn1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationclathrin binding middle part aa 144-575PromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199204PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-intDepositorInsertT2A-split-intein-Gal4[21-881]-C-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD406
Plasmid#163111PurposeExpression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mNeonGreen_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
