166,485 results

1. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGFP Cortactin

   Plasmid

   #50728

   Purpose

   mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cortactin (CTTN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA560

   Plasmid

   #186236

   Purpose

   pTet-eLbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Wnt promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EWB-DIO-cyan pre-eGRASP(p32)

   Plasmid

   #111589

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p32)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7

   Plasmid

   #214812

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-jGCaMP8f-WPRE (AAV Retrograde)

   Viral Prep

   #179254-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Tags

   None

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

9. enIscB

   Plasmid

   #205410

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid

    #102898

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5guides-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynap.iGABASnFR

    Plasmid

    #112159

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pDDGFP_LEU2D

    Plasmid

    #58352

    Purpose

    Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

    Depositor

    Simon Newstead

    Article

    Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-tdTomato (codon diversified) (AAV9)

    Viral Prep

    #59462-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (codon diversified)

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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