We narrowed to 3,510 results for: Braf;

1. pUFlip-floxed2A-KalTA4-; gcry1:BFP -0

   Plasmid

   #173889

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

   Plasmid

   #173890

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

   Plasmid

   #173891

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-ubb-Avi-Cerulean-Rpl10a

   Plasmid

   #79882

   Purpose

   Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTB2-Avi-Cerulean-Rpl10a

   Plasmid

   #79880

   Purpose

   Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT22gcfUbm5En25EE4PCh(#552)

   Plasmid

   #184092

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   5myc-chkEn2(5E)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT22Ubm4Eng2bKRPC(#493)

   Plasmid

   #184091

   Purpose

   cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   4xMyc

   Expression

   Mutation

   ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Expression

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3TS-zSpRY

   Plasmid

   #179317

   Purpose

   In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Expression

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-HuC(elavl3)-CaMPARI2

    Plasmid

    #137185

    Purpose

    pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
    

    Use

    Tol2 plasmid for zebrafish

    Tags

    FLAG, HA, myc

    Expression

    Mutation

    Promoter

    HuC(elavl3)

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2CG2-ubi:QFGal4-SV40pA

    Plasmid

    #155119

    Purpose

    Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    ubi:QFGal4 (ubb Zebrafish, Budding Yeast, Neurospora crassa)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    ubiquitin B

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2-UAS-zmiRFP

    Plasmid

    #108415

    Purpose

    Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zmiRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid

    #230043

    Purpose

    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-UAS-zArchon1-KGC-EGFP-ER2

    Plasmid

    #108427

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon1-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-UAS-zArchon2-KGC-EGFP-ER2

    Plasmid

    #108428

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon2-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R] (Recombinant Antibody)

    Antibody

    #182998-rAb

    Purpose

    Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Recommended Applications

    Immunohistochemistry and Western Blot

    Reactivity

    Human, Mouse, Rat, and Zebrafish

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available since

    May 31, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

17. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSYC-201

    Plasmid

    #178963

    Purpose

    Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Inserts

    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    

    Use

    Tol2 mediated recombination

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    zebrafish gfap promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-CASK [K56A/50R] (Recombinant Antibody)

    Antibody

    #183002-rAb

    Purpose

    Anti-CASK (Human) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Recommended Applications

    Immunohistochemistry and Western Blot

    Reactivity

    Frog, Human, Mouse, Rat, and Zebrafish

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available since

    May 31, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

20. pSYC-204

    Plasmid

    #178964

    Purpose

    Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Inserts

    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    

    Use

    Tol2 mediated recombination

    Tags

    3xFLAG

    Expression

    Mutation

    R79C

    Promoter

    Zebrafish gfap promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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