We narrowed to 12,623 results for: cas9 plasmid

1. Nsp2Cas9_AAV

   Plasmid

   #192143

   Purpose

   Expresses Nsp2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NarCas9_AAV

   Plasmid

   #192145

   Purpose

   Expresses NarCas9?and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   NarCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

   Plasmid

   #127908

   Purpose

   WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

   Depositor

   Mark Groudine

   Insert

   gRNA for Human 5' HP1beta
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS1089: mini-AAV.sgRNA.Nme2Cas9

   Plasmid

   #159536

   Purpose

   Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 with single guide RNA cassette
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

   Plasmid

   #127560

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   EGFP-D102G see depositor comments below

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p-dCas9-JMJD2A-Hygro

   Plasmid

   #104410

   Purpose

   transient expression of dCas9-JMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   JMJD2A (KDM4A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p-dCas9-G9a-Hygro

   Plasmid

   #104408

   Purpose

   transient expression of dCas9-G9a fusion protein

   Depositor

   Stephan Beck

   Insert

   G9a (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p-dCas9-p300-Hygro

   Plasmid

   #104411

   Purpose

   transient expression of dCas9-p300 fusion protein

   Depositor

   Stephan Beck

   Insert

   p300 (EP300 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-dCas9-DNMT3a-Hygro

    Plasmid

    #104403

    Purpose

    transient expression of dCas9-DNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    DNMT3a (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZ8-T_dCas9

    Plasmid

    #74062

    Purpose

    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJWV102-wtcas9sp

    Plasmid

    #99612

    Purpose

    Plasmid containing wild-type cas9sp

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    cas9sp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PczcD

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEVA47_dCas9-GFP_NOT_i1

    Plasmid

    #231416

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124287

    Purpose

    sgRNA against human Col7A1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

18. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124286

    Purpose

    sgRNA against human Col7A1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge