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pAcGFP-N1_CBPdIDR7KTG

Plasmid

#221915

Purpose

Transient expression of GFP-tagged CBPdeltaIDR7KTG in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR7 (aa 2109-2434); mutation of lysi…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M5

Plasmid

#204563

Purpose

Expresses GST-tagged UFBP1 M5 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

215-314aa

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EDC3

Plasmid

#155561

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EDC3 (EDC3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M3

Plasmid

#204561

Purpose

Expresses GST-tagged UFBP1 M3 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

116-314aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M4

Plasmid

#204562

Purpose

Expresses GST-tagged UFBP1 M4 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

116-214aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 F196A V198A

Plasmid

#204564

Purpose

Expresses GST-tagged UFBP1 F196A V198A in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

F196A V198A

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-gephyrin S268E/270E

Plasmid

#199609

Purpose

Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

S268E/S270E

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3 K214R

Plasmid

#204570

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

K214R

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3

Plasmid

#204569

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

none

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-GABARAP

Plasmid

#204574

Purpose

Expresses Ash-tagged GABARAP in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Mutation

deltaL117

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB Ubiqutin-BFP

Plasmid

#202426

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

Ubiquitin, TagBFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

Plasmid

#148514

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T

Plasmid

#147590

Purpose

Insect Expression of DmThor-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CG32016-E (Thor Fly)

Expression

Insect

Mutation

DmThor two silent mutations compared to the seque…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T

Plasmid

#147591

Purpose

Insect Expression of DmThor-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmCup_417-1133 (Thor Fly)

Expression

Insect

Mutation

Thor contains two silent mutations A246C and T321…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T

Plasmid

#147592

Purpose

Insect Expression of DmThor-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmeIF4Ga_687-1666 (Thor Fly)

Expression

Insect

Mutation

DmThor two silent mutations A246C and T321C compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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