We narrowed to 53,085 results for: Eng

1. pSi-check2-zRac2 - mut 3'UTR

   Plasmid

   #97159

   Purpose

   For dual luciferase report assay

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dre-Rac2 3'UTR
   

   Expression

   Mammalian

   Mutation

   miR-722 Binding Mutant

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSi-check2-zRac2 3'UTR

   Plasmid

   #97158

   Purpose

   For dual luciferase report assay

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dre-Rac2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFPB6

   Plasmid

   #83557

   Purpose

   Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL10 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TU#1412_tiam-1_cDNA

   Plasmid

   #82364

   Purpose

   express tiam-1 cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   tiam-1 cDNA (tiam-1 Nematode)
   

   Expression

   Worm

   Promoter

   none

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TU#1449_unc-73e_cDNA

   Plasmid

   #82363

   Purpose

   express unc-73e cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-73 e isoform (unc-73 Nematode)
   

   Expression

   Worm

   Promoter

   none

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD

   Plasmid

   #73303

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Tags

   intein

   Expression

   Bacterial

   Mutation

   E1083Q

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

   Plasmid

   #73299

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Tags

   intein

   Expression

   Bacterial

   Mutation

   S1079N-E1083Q

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pTH1-SaSrtA-KQ

   Plasmid

   #64982

   Purpose

   Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108Q

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. human pirh2-(138-261)

   Plasmid

   #24882

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   Pirh2 (RCHY1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains aa 138-261.

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

10. human pirh2-(187-261)

    Plasmid

    #24883

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 187-261.

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

11. L1-W1036L

    Plasmid

    #15488

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-W1036L (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Tryptophan 1036 changed to Leucine

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pANY4-pL18-ccdB

    Plasmid

    #164898

    Purpose

    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-12x

    Plasmid

    #61699

    Purpose

    Expresses PTDH mutant Opt12x

    Depositor

    Huimin Zhao

    Article

    Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

    Insert

    Opt12x
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pANY4-pR18/pL18-ccdB

    Plasmid

    #164899

    Purpose

    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJKW 0633

    Plasmid

    #74122

    Purpose

    Photinus pyralis sulfotransferase ST2 - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis sulfotransferase 2
    

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CoV2-Spike-D614G-Alpha

    Plasmid

    #177961

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    

    Expression

    Mammalian

    Mutation

    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Human, Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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