We narrowed to 16,198 results for: grna

1. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

   Plasmid

   #67990

   Purpose

   CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SMVP-Cas9N-U6-gRNA P1

   Plasmid

   #80943

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6-gRNA-TnT-Cre

   Plasmid

   #132551

   Purpose

   For loss-of-function experiments in cardiomyocytes

   Depositor

   William Pu

   Inserts

   TnT-Cre
   U6-gRNA
   

   Use

   AAV

   Promoter

   TnT and U6

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

   Plasmid

   #211966

   Purpose

   Express gRNA against JARID2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting JARID2 (JARID2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB700 lenti gRNA Blasto_zhang2.0

   Plasmid

   #167912

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2-TRAF6-targeting sgRNA 1

   Plasmid

   #131345

   Purpose

   gRNA targeting mouse TRAF6

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   gRNA targeting mouse TRAF6 (Traf6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP109 pEF1a-gRNA-GFP

   Plasmid

   #100935

   Purpose

   GFP, puromycin and gRNA scaffold driven by EF1a promoter

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   gRNA scaffold
   

   Use

   Grna scaffold

   Expression

   Mammalian

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

    Plasmid

    #129530

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. plentiCRISPRv2-PURO-LYSET-gRNA1

    Plasmid

    #210489

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA1 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCRISPRv2-PURO-LYSET-gRNA2

    Plasmid

    #210490

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA2 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB700 lenti gRNA Hygro

    Plasmid

    #167907

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. B2M Bulldozer (gRNA crB2M_13)

    Plasmid

    #84381

    Purpose

    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells

    Depositor

    Chad Cowan

    Article

    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.

    Insert

    B2M Bulldozer crB2M_13 gRNA
    

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CR7T

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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