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Showing: 4841 - 4860 of 160228 results

  4. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid
    #101785
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Insert
    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101785-plasmid-map-sequence-id-197676

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  5. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

    Viral Prep
    #26971-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. TFORF2671

    Plasmid
    #142972
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NEUROD4 (NEUROD4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142972-plasmid-map-sequence-id-397310

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  9. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  10. pFLAG-CMV-hErk1(K71R)

    Plasmid
    #49329
    Purpose
    Expresses kinase deficient FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K71R
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. AICSDP-26:ST6GAL1-mEGFP

    Plasmid
    #101786
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1
    Depositor
    Allen Institute for Cell Science
    Insert
    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101786-plasmid-map-sequence-id-197675

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  14. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  15. pMAH2-CageCys

    Plasmid
    #71404
    Purpose
    Expresses tRNA and synthetase for mammalian encoding of a photocaged cysteine
    Depositor
    Huiwang Ai
    Article
    Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8
    Insert
    aminoacyl-tRNA synthetase for a caged cysteine
    Use
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  20. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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Showing: 4841 - 4860 of 160228 results