167,318 results

1. pGT-B1

   Plasmid

   #122579

   Purpose

   MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1 gfaABC1D-Glt1-eGFP

   Plasmid

   #176862

   Purpose

   Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGEN-dPspCas13b-NES-mCherry

   Plasmid

   #233609

   Purpose

   Expression of catalytically-inactive PspCas13b in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Mutation

   H133A/H1058A

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubc.Luc.IRES.Puro

   Plasmid

   #33307

   Depositor

   Linzhao Cheng

   Article

   Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGT-Ah9

   Plasmid

   #122576

   Purpose

   MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   tetQ/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. USP15

   Plasmid

   #28282

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15 (USP15 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Contains dusp and Ubl domains (residues 6-223).

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. Human ubiquitination-related proteins CRISPR KO library

   Pooled Library

   #174592

   Purpose

   CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.

   Depositor

   Juan Bonifacino

   Article

   Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. MSCB-hDot1Lmut

    Plasmid

    #74174

    Purpose

    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    hDot1L 163 GSG to RCR mutation*

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225241

    Purpose

    Mammalian expression of iPASnFR-H in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Rab7a-7

    Plasmid

    #55127

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO GFP11-LRRK2_G2019S

    Plasmid

    #231175

    Purpose

    Expresses GFP-11-LRRK2 G2019S in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (G2019S) (LRRK2 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169283

    Purpose

    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Tet1

    Plasmid

    #60938

    Purpose

    Expresses Tet1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+mScarlet3-C Cloning Vector

    Plasmid

    #220616

    Purpose

    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    mScarlet3 fusion

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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