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pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

Plasmid

#176587

Purpose

Bacterial expression of His14-MBP-TEV-AmaTnpB

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

His14 tag

Expression

Bacterial

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1583

Plasmid

#121054

Purpose

GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized GFP, and auxin…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTD103-ARG1

Plasmid

#106475

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYLCRISPR/Cas9pUbi-N

Plasmid

#106331

Purpose

Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

CRISPR

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

ZmUbi

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTECH-MbPylRS(IPYE)

Plasmid

#104072

Purpose

Expression plasmid encoding MbPylRS(IPYE) and pylT

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MbPylRS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Publication

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJ1996_v2

Plasmid

#140664

Purpose

dCas9 & Csy4

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9 & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Bsrs103-RpaR-RpaI

Plasmid

#85159

Purpose

Constitutively expresses RpaR and inducibly expresses RpaI

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Insert

RpaR, RpaI

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Publication

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pICH41744

Plasmid

#48017

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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(attB) tdMCP-tagged BRaf with MS2-circRNA

Plasmid

#226163

Purpose

Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

3xFlag-tdMCP-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP

Expression

Mammalian

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ136A

Plasmid

#69278

Purpose

Golden Gate entry vector; 6th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

Plasmid

#117168

Purpose

CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

Plasmid

#89570

Purpose

Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

Depositor

McLean Bolton

Publication

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Inserts

Channelrhodopsin
Histone 2B

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFC331

Plasmid

#87843

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-Flag-Mm Ezh2 Y641F

Plasmid

#80077

Purpose

Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

Depositor

Phillip Abbosh

Inserts

Flag-EZH2 Y641F (Ezh2 Mouse)
Hygromycin phosphotransferase

Use

Retroviral

Tags

5' FLAG

Expression

Mammalian

Mutation

Y641F, R342R, N393N

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYTK067

Plasmid

#65174

Purpose

Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConR1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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