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pRN3P-HA-Suv4-20h1mut

Plasmid

#86690

Purpose

For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)

Tags

HA

Mutation

mutated region NHDC (asparagine 273 to cysteine 2…

Promoter

T3

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6WT

Plasmid

#46781

Purpose

expression of WT Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 (RAB6A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_WT

Plasmid

#208770

Purpose

Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p205-5'-Switch-ON

Plasmid

#217884

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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CoV2-N-T205I

Plasmid

#177954

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (T205I) (N )

Expression

Mammalian

Mutation

T205I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C(E184Q)-HA

Plasmid

#209251

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Tags

HA

Expression

Mammalian

Mutation

E184Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8A1(E191Q)-HA

Plasmid

#209240

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Tags

HA

Expression

Mammalian

Mutation

E191Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-DN-REST

Plasmid

#61533

Purpose

Expresses DN-REST in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

REST (REST Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P73, Dominant negative REST (contains the Zf DNA-…

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8 Flag2AUF1(p37)

Plasmid

#135944

Purpose

Expresses AUF1p37 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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VRQR variant

Plasmid

#138562

Purpose

Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335Q, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CathepsinG-CD4d3+4-bio

Plasmid

#73125

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CathepsinG with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Cathepsin G (CTSG Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-HDAC4 3SA

Plasmid

#45637

Depositor

Tso-Pang Yao

Article

Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

Insert

HDAC4 (HDAC4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

3SA

Promoter

CMV

Available Since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_eCB2.0

Plasmid

#164609

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

GfaABC1D

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-bHs

Plasmid

#134259

Purpose

For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

Depositor

Edwin Manuel

Article

Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

Insert

Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium

Tags

6x His

Expression

Bacterial

Mutation

codon optimized for Salmonella typhimurium

Promoter

AraC

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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