We narrowed to 9,543 results for: pho;
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Plasmid#59826PurposeAllows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationNon-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)
Plasmid#192444PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N
Plasmid#22018DepositorInsertmVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseRetroviralExpressionMammalianMutationRac1 starts at I4 and contains mutation T17N.Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GP1Fc Cl13
Plasmid#138599PurposeExpresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)DepositorInsertLCMV GP1-Fc
TagsFc fusion proteinExpressionMammalianPromoterCMVAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO CPTP
Plasmid#170735PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH726-CEN-RLuc/minCFLuc
Plasmid#38210DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Fzd6
Plasmid#123588DepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro HA-Atg14*dCC
Plasmid#38266DepositorInsertautophagy related genes 14 (ATG14 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged A618T, A621T, C624A and T627C for siRNA r…PromoterLTRAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-CLCb (EED/QQN)
Plasmid#47422DepositorInsertCLCb EED/QQN (CLTB Human)
TagsEGFPExpressionMammalianMutationEED changed to QQN, in aa20-41 regionPromoterCMVAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HA-Gnat1 WT-3UTR
Plasmid#184026PurposeExpresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTRDepositorAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG human Bmf
Plasmid#17242DepositorAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-HK2_sgRNA2
Plasmid#201597PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertHK2 (HK2 Human)
UseCRISPR and LentiviralAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2
Plasmid#107730PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1
Plasmid#107729PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP2A-FBAR-mRFP
Plasmid#168502PurposeExpression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.DepositorAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-dKARD
Plasmid#114053Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-dKARD (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance): AGATACTAGCT…Available SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG mouse Bmf
Plasmid#17243DepositorAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181E
Plasmid#105312PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181A
Plasmid#105307PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGK-hMIGA2-DsRed
Plasmid#192867PurposeExpress codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
TagsDsRedExpressionMammalianPromoterPGKAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-GOLPH3
Plasmid#21688DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseGateway entry cloneMutationlast nucleotide of stop codon removed for c-termi…Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFLAG R106L-CPTP
Plasmid#170744PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG K60A-CPTP
Plasmid#170743PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ly6G-miR30
Plasmid#163358PurposeLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.DepositorAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMY-Ly6G-Rluc_miR
Plasmid#163339PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface markerDepositorAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase
Plasmid#75018PurposeEncodes the expression of Luciferase (with out any fluorescent reporter)DepositorInsertfirefly Luciferase
UseLuciferase and RetroviralAvailable SinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only