We narrowed to 7,375 results for: ef1a

1. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-DHB-mTurq2

   Plasmid

   #212667

   Purpose

   Express tagged DHB

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   DHB (HELB Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-EF1alpha-scFv-VP64_Blast

   Plasmid

   #192655

   Purpose

   3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-flex-TVA

   Plasmid

   #69618

   Purpose

   Express recepter TVA for envA with AAV

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

   Insert

   Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ef1a-DIO-eGCaMP(2+)

   Plasmid

   #201150

   Purpose

   AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

   Depositor

   Andre Berndt

   Article

   Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

   Insert

   eGCaMP_2+
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-EF1a-mTagRFP-T-CAAX

   Plasmid

   #167973

   Purpose

   AAVS1 targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFP-T-CAAX

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1a-extGluc-NGFR

   Plasmid

   #104832

   Purpose

   extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   extGLuc
   NGFR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EF1A-DIO-mRuby2

   Plasmid

   #126215

   Purpose

   Labeling of cre-expressing neurons (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. IF101: pMVP (L1-L4) EF1a promoter

    Plasmid

    #121687

    Purpose

    pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-DIO-HTB

    Plasmid

    #44187

    Depositor

    Edward Callaway

    Insert

    Histone tagged GFP, T2A-TVA-E2A-B19G
    

    Use

    AAV; Adeno-associated vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pPB-EF1a-BCL2-hygro

    Plasmid

    #222581

    Purpose

    PiggyBac transposon plasmid for constitutive expression of BCL2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    BCL2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-PGD2-1.1

    Plasmid

    #214764

    Purpose

    AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF-1-alpha promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-TALEN_HD (SPC right)

    Plasmid

    #126695

    Purpose

    right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC right
    

    Use

    TALEN

    Tags

    NLS, truncated N term and TAL, FokI

    Expression

    Mammalian

    Mutation

    R451P in SPC

    Promoter

    EF1

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a-TALEN_NN (SPC left)

    Plasmid

    #126694

    Purpose

    left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC left
    

    Use

    TALEN

    Tags

    0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. Lv EF1a MS2-Fkbp1x 2A Hygro

    Plasmid

    #102806

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pKLV2-EF1a-GFP ArgSen

    Plasmid

    #184695

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. EF1a CHR2 mCherry WPRE

    Plasmid

    #129432

    Purpose

    expression of CHR2 mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    CHR2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV Ef1a-mRuby3-WPRE

    Plasmid

    #135427

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid

    #200676

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFIN-EF1a-GFP-WPRE

    Plasmid

    #44352

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEnt L1L3 EF1a-tTA-3

    Plasmid

    #24415

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV-EF1aGFP2AKAT7-linker-mAID-W

    Plasmid

    #159294

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 (KAT7 Human)
    

    Use

    Lentiviral

    Tags

    mAID (auxin-inducible degron)-tagged

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10)

    Promoter

    human EF1a promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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