We narrowed to 9,156 results for: pcas

1. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available Since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-B19M

   Plasmid

   #59923

   Purpose

   Expression vector for Rabies virus SAD B19 matrix protein gene

   Depositor

   Ian Wickersham

   Insert

   Rabies virus matrix protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-EnvARGCD

   Plasmid

   #100800

   Purpose

   express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain

   Depositor

   Ian Wickersham

   Insert

   fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-tdTHC

   Plasmid

   #112621

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-6020nes

   Plasmid

   #108656

   Purpose

   A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-JNK-EV
   

   Tags

   XFP

   Expression

   Mammalian

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GFPmG1

   Plasmid

   #50444

   Purpose

   Expression of a EGFP variant in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GFPmG1
   

   Expression

   Mammalian

   Mutation

   E143K, N147Q

   Promoter

   CAG

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas_5+7_gfp

   Plasmid

   #60595

   Purpose

   encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_5+7_gfp
   

   Expression

   Bacterial

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-ROSAf

   Plasmid

   #54323

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-VHC

   Plasmid

   #112618

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaRNA3-eIFiso4E120(+)

    Plasmid

    #86092

    Purpose

    This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    eIFiso4E
    

    Expression

    Plant

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Bmp5

    Plasmid

    #163589

    Purpose

    Expresses BMP-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp5 (Bmp5 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCAGGS-ROSAr

    Plasmid

    #54324

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Expression

    Mammalian

    Promoter

    CAGG

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-i53

    Plasmid

    #119002

    Purpose

    Mammalian expression of i53 (53BP1) inhibitor and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    i53
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAD104_Asn152Ser_pCMV6Entry-hIRG1

    Plasmid

    #124877

    Purpose

    Expresses hCAD Asp152Ser mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Asn152 to Ser

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP-PCAF-C2

    Plasmid

    #134572

    Purpose

    Expresses YFP-PCAF fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    PCAF (Kat2b Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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