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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9961 - 10000 of 23042 results

  2. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  9. pET24a-VHH-std

    Plasmid
    #109417
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109417-plasmid-map-sequence-id-218403

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  17. ZJOM135

    Plasmid
    #133659
    Purpose
    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133659-plasmid-map-sequence-id-265905

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  20. bNS2B47NS3

    Plasmid
    #155320
    Purpose
    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155320-plasmid-map-sequence-id-318477

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  23. pcDNA3-mNeptune2-N

    Plasmid
    #41645
    Purpose
    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    mNeptune2-Dronpa145N
    Expression
    Mammalian
    Mutation
    K145N on Dronpa (allows control of photochromic F…
    Promoter
    CMV
    Available Since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41645-plasmid-map-sequence-id-296256

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  29. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

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  37. pET28a Gx-NL

    Plasmid
    #169105
    Purpose
    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169105-plasmid-map-sequence-id-335658

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  38. ZJOM143

    Plasmid
    #133667
    Purpose
    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133667-plasmid-map-sequence-id-265896

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  39. pSNK

    Plasmid
    #135193
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135193-plasmid-map-sequence-id-288121

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Showing: 9961 - 10000 of 23042 results