We narrowed to 9,802 results for: otos

1. pICE-EGFP-FLAG-PSMD2

   Plasmid

   #161917

   Purpose

   Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

   Plasmid

   #84481

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

   Plasmid

   #179465

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P-Kv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-JEDI-2P-Kv-WPRE

   Plasmid

   #179463

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV9

   Insert

   JEDI-2P-Kv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Syn-Chronos-tdTomato

   Plasmid

   #62726

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Epac-S-H189

   Plasmid

   #170350

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-Mito-DsRed1

   Plasmid

   #87379

   Purpose

   Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

   Depositor

   Björn Stork

   Article

   Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

   Insert

   TXN2 mitochondrial transit peptide (TXN2 Human)
   

   Use

   Retroviral

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   First 60 aa only

   Promoter

   Retroviral 5' LTR promoter

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #130909

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CaMKII-ChrimsonR-tdTomato

   Plasmid

   #99231

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CaMKII

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84484

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #84480

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-FLEX-rc[Chronos-GFP]

    Plasmid

    #62722

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Syn-FLEX-rc[CoChR-GFP]

    Plasmid

    #62724

    Purpose

    AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CoChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-Tet-On-HTR6-RhoA sensor

    Plasmid

    #189614

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6 (HTR6 Human)
    

    Use

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Tet3G

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-mFgf21pro2-luc

    Plasmid

    #101798

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-835_NY-ESO-1_TCR_FAS/MyD88

    Plasmid

    #207498

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/MyD88, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-mFgf21pro3-luc

    Plasmid

    #101799

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Use

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV.CAG-FLEX.iAChSnFR-Venus

    Plasmid

    #137959

    Purpose

    Expresses FLEXed iAChSnFR (yellow version) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (yellow variant)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.CAG.iAChSnFR-NULL

    Plasmid

    #137956

    Purpose

    Expresses iAChSnFR (non-binding variant) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding variant)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Y140A binding site mutant

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-1BPNLS-Cas9-1BPNLS

    Plasmid

    #87108

    Purpose

    BPNLS-Cas9-BPNLS expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-GFAP-JEDI-2P-WPRE

    Plasmid

    #179469

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSBbi-moxGFP-P2A-T2A-Puro

    Plasmid

    #176893

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A
    

    Use

    Transposon

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIG-832_NY-ESO-1_TCR_FAS/4-1BB

    Plasmid

    #207495

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/4-1BB, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/Puro-CAG-JEDI-2P-Kv

    Plasmid

    #179462

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P-Kv
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128589

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    Syn

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIG-834_NY-ESO-1_TCR_FAS/IL4R

    Plasmid

    #207497

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/IL4R, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

    Plasmid

    #207487

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge