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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKO_Luciferase_sgRNA

Plasmid

#74190

Purpose

lentiviral vector expressing sgRNA targeting Luciferase

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Luciferase sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-FGFR4

Plasmid

#60531

Purpose

Entry vector for human FGFR4

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

FGFR4 (FGFR4 Human)

Use

Entry vector

Mutation

c2134a P712T

Promoter

NA

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mDlx-BiPOLES-mCerulean

Plasmid

#154946

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

minimal Dlx

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2232

Plasmid

#158401

Purpose

High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple316 (NR2E1 Human)

Use

Unspecified

Promoter

Ple316

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ATG-1929

Plasmid

#108712

Purpose

CBR2opt in pF4Ag expression vector with T7 and CMV promoter

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt

Expression

Bacterial and Mammalian

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

T7, CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Cterminal HA Mios pRK5

Plasmid

#46329

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
409 pSG5L HA RB (379-928)

Plasmid

#10734

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (379-928) (RB1 Human)

Expression

Mammalian

Mutation

amino acids 379-928

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-hIes2

Plasmid

#15355

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

PAP-1 binding protein (INO80B Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pENTR1A hu_ncOGT WT

Plasmid

#154281

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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TCR012H

Plasmid

#233454

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR012H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR012H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

Plasmid

#47458

Purpose

LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS2214

Plasmid

#158417

Purpose

AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple265 (PCP2 Human)

Use

AAV

Promoter

Ple265

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pLJM1

Plasmid

#46337

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Lentiviral

Tags

FLag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-delta133HtrA-EGFP

Plasmid

#14124

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

Insert

HtrA2 (HTRA2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 133 amino-acids

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-MCS-shCHD5 #2

Plasmid

#68877

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only