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MBP-hnRNPA2_deltaLC

Plasmid

#98664

Purpose

Expresses MBP-tagged hnRNPA2 without the LC domain

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

G190Stop

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero20

Plasmid

#187930

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero20

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero20 (C11orf58 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-AR-R405A

Plasmid

#126052

Purpose

Expresses human androgen receptor (R405A) in mammalian cells

Depositor

Elizabeth Wilson

Article

Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

Insert

AR (AR Human)

Expression

Mammalian

Mutation

R405A

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short W284A

Plasmid

#72551

Purpose

expresses 3*FLAG tagged human NSD3-short with W284A mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

W284A

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL-C144A

Plasmid

#160285

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86020

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine, threonine 230 to al…

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_D290V

Plasmid

#98663

Purpose

Expresses MBP-tagged full length hnRNPA2 with D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Ovtm

Plasmid

#51888

Purpose

expression vectore in mammalian cells

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mFbxl3 (Fbxl3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

ovtm (I364T)

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
Desmoplakin II tension sensor (FL-based)

Plasmid

#118721

Purpose

The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

inserted FL-based tension sensor module after aa1…

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594V

Plasmid

#131715

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594V

Promoter

CMV

Available Since

May 27, 2023

Availability

Academic Institutions and Nonprofits only

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BTG3

Plasmid

#155427

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG3 (BTG3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666A

Plasmid

#105310

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600K

Plasmid

#131720

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600K

Promoter

CMV

Available Since

Dec. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pTagGFP2-N SIN1 delta2-29

Plasmid

#124923

Purpose

For expression of a.a.2-29 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86023

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed threonine 230 to…

Promoter

CMV IE

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available Since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AR-R405S

Plasmid

#126051

Purpose

Expresses human androgen receptor (R405S) in mammalian cells

Depositor

Elizabeth Wilson

Article

Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

Insert

AR (AR Human)

Expression

Mammalian

Mutation

R405S

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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