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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E14: attB4-EF1p>-attB5

Plasmid

#162975

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pEPYCdKN0001

Plasmid

#196148

Purpose

Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

9xHis_AtTGA1_HiBit

Expression

Bacterial

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4AD

Plasmid

#26255

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VP64

Plasmid

#79375

Purpose

Recruits VP64 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGC630

Plasmid

#107013

Purpose

GFP reporter of lag-2 driven by 2 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Expression

Worm

Promoter

lag-2

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pN_35S/mApple

Plasmid

#132566

Purpose

mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mApple

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Article

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Expression

Yeast

Promoter

PMA1

Available Since

Feb. 4, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC-9-AtHSP-11

Plasmid

#197760

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtHSP terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_D5802

Plasmid

#176880

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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mRuby2-NT-C1

Plasmid

#54561

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2-NT

Expression

Mammalian

Available Since

June 22, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET15b-At 14-3-3 epsilon

Plasmid

#44936

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 epsilon (GRF10 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pLBT0190

Plasmid

#172815

Purpose

Level 1 module containing OEP7-mTRQ_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-OEP7-mTRQ-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNL2080 pGATA23 GG0-GG1

Plasmid

#195908

Purpose

Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-AFB2

Plasmid

#83845

Purpose

Gateway donor vector for AFB2

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

AFB2 (AFB2 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paCRY2(D387A)

Plasmid

#129652

Purpose

2P-activatable CRY2 with the FAD-disrupting mutation

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2(D387A)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMT440

Plasmid

#8609

Depositor

Robert Hartley

Article

Yazynin et al Gene 1996 Feb 22;169(1):131-2.

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

V36 codon of barnase of pMT416 replaced by polyli…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only