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Plasmid#209776PurposeCRISPRi for STK4DepositorInsertNluc, sgSTK4i, mRuby2 (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A
Plasmid#204622PurposeSingle-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.DepositorInsertmRFP1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-C1-crRNA-cloning-EF1a-BA
Plasmid#204623PurposepiggyBac vector for crRNA cloning and expression. Blasticidin selectable.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-Oct4
Plasmid#197909Purposesingle-particle tracking for OCT4DepositorInsertpou5f1 (Pou5f1 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a::DIO-iLMO2
Plasmid#129482PurposeAAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-Aion-citrine
Plasmid#192571PurposeAAV-vector for optogenetic long-term silencing of Cre-positive neuronsDepositorInsertAion
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…PromoterEf1-alphaAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-somAion-citrine
Plasmid#192573PurposeAAV-vector for optogenetic long-term silencing of Cre-positive neuronsDepositorInsertsomAion
UseAAVTagsCitrine - soma-targeting motif from Kv2.1 channelExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…PromoterEf1-alphaAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP RanSen
Plasmid#184697PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)NLS
Plasmid#184689PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen (-)NLS
Plasmid#184698PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Mito-pHCountdown
Plasmid#182302Purposelentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selectionDepositorInsertmito pH countdown
UseLentiviralTagsCOX8A mitochondria targeting sequenceExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmClover3-hsLem2
Plasmid#179524PurposeExpress OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoterDepositorInsertLEMD2 (LEMD2 Human)
UseTagsmClover3-mAIDExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-CHMP3(147)-HA
Plasmid#154177Purposeexpresses mouse CHMP3(147) in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 147PromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4(TA)-ires-blast
Plasmid#167831PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1024 EF1alpha TetON3G in TU5
Plasmid#139261PurposeExpresses TetON3G under hEF1alpha in TUPV5DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM469 EF1alpha TetON3G in TU4
Plasmid#139260PurposeExpresses TetON3G under hEF1alpha in TUPV4DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM422 EF1alpha TetON3G in TU3
Plasmid#139259PurposeExpresses TetON3G under hEF1alpha in TUPV3DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim EF1 MCH (CEM)
Plasmid#104444PurposeExpresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoterEF1Available sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo
Plasmid#128454Purposelentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA601: pMVP (L3-L2) pA; EF1a::TETa-pA
Plasmid#121805PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY704: pMVP (L1-R5) EF1a promoter
Plasmid#121693PurposepMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Sox2TALE-GCN4
Plasmid#120548PurposeExpress 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 geneDepositorInsertNLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-NGN2TALE-GCN4
Plasmid#120547PurposeExpress 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 eneDepositorInsertNLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-R310_RASA2-EF1-Puro
Plasmid#114097PurposeExpress mutant RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR310*PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV40-EF1a-hBCL10(1-114)-SO
Plasmid#75257Purposeretroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)DepositorInsertBcl10 (BCL10 Human)
UseRetroviralTagsStrepOne TagExpressionMammalianMutationAS 1-116 (CARD-domain)PromoterpMSCV-LTRs, EF1aAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Fon-copGFP
Plasmid#72260PurposeExpress copGFP when FLP is expressed by another vectorDepositorInsertcopGFP
UseLentiviralTagsExpressionMammalianMutationPromoterelongation factor-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-2TK-WW-EF1+EF2
Plasmid#18080DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ptef1-CSC-Tpdc1
Plasmid#127739PurposeYeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
Plasmid#196196Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
UseHomology repair donorTagsV5-APEX2-mScarlet-IExpressionMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only