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We narrowed to 15,998 results for: grn

Showing: 501 - 520 of 15998 results

  2. AY27_pU6.gRNA.CLYBL

    Plasmid
    #199238
    Purpose
    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    S. pyogenes gRNA spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199238-plasmid-map-sequence-id-391387

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  5. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Article
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52255-plasmid-map-sequence-id-80847

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  11. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  14. p426_Cas9_gRNA-CAN1y

    Plasmid
    #87391
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87391-plasmid-map-sequence-id-193434

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  15. pUC57kan-T7-gRNA-U6 V2

    Plasmid
    #115520
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Xingxu Huang
    Insert
    sgRNA scaffolf-U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    115520-plasmid-map-sequence-id-224556

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Showing: 501 - 520 of 15998 results