167,468 results

1. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Hisx6

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSOD1-FLAG

   Plasmid

   #182922

   Purpose

   Mammalian expression plasmid for human WT SOD1

   Depositor

   Philip Eaton

   Article

   Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

   Insert

   [Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available Since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 Control Vector

   Plasmid

   #212937

   Purpose

   Control vector with constitutive firefly luciferase expression

   Depositor

   Debrya Groskreutz

   Insert

   luc+
   

   Use

   Luciferase

   Mutation

   WT

   Promoter

   SV40

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pPM47 (UPR-RFP CEN/ARS URA3)

   Plasmid

   #20132

   Depositor

   Feroz Papa

   Article

   Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

   Insert

   UPR-RFP
   

   Expression

   Yeast

   Available Since

   Feb. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG:GPI-GFP

   Plasmid

   #32601

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Rhee et al Genesis. 2006 Apr;44(4):202-18.

   Insert

   GPI-GFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSTARR-seq_ORI vector

   Plasmid

   #99296

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

   Plasmid

   #176851

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1-5SA (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. evoFERNY-Cas9NG

    Plasmid

    #203329

    Purpose

    Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    evoFERNY-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-TetON-Sox2

    Plasmid

    #110280

    Purpose

    Doxycycline-inducible overexpression of Sox2

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    No fusion genes

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    TRE

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABEpuro mTurquoise2 LC3B

    Plasmid

    #78518

    Purpose

    Fluorescent reporter for LC3B visualization

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    retroviral

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EMMA toolkit

    Plasmid Kit

    #1000000119

    Purpose

    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

    Depositor

    Yizhi Cai

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-CSF1R-V5/HIS

    Plasmid

    #201984

    Purpose

    expression of human CSF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    CSF1R receptor tyrosine kinase (CSF1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-Casp8

    Plasmid

    #11817

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 (CASP8 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET-30-His6-SUMO-AEY-CfaN

    Plasmid

    #122949

    Purpose

    Bacterial expression of N-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaN
    

    Expression

    Bacterial

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSEVA63-GG

    Plasmid

    #208015

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-EF1a-fDIO-Cre (AAV5)

    Viral Prep

    #121675-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

27. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHM131

    Plasmid

    #225530

    Purpose

    Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    mCherry
    BFP
    

    Expression

    Mammalian

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-LC3 (human)

    Plasmid

    #24920

    Purpose

    Mammalian expression of LC3 fused to EGFP

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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