167,785 results
-
-
pLVX-M-Flag-BAP1
Plasmid#125840Purposeexpress wild type BAP1DepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W
Plasmid#67981PurposeCas9 activity reporter (control) with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPREAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9
Plasmid Kit#1000000154PurposeVectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.DepositorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Arl13b-mCardinal
Plasmid#194542PurposeExpresses Arl13b with mCardinal in mammalian cells and zebrafish embryosDepositorAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGluSnFR4s-NGR-WPRE
Plasmid#234451PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterCAGAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated optoDroplet
Plasmid#111507PurposeFor membrane localized optoDropletsDepositorInsertCry2 (CRY2 Mustard Weed)
UseLentiviralTagsFUS, Fusion Red, and Myristoylation Sequence (MYR…PromoterSFFVAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LAMP1
Plasmid#110948PurposeExpresses LAMP1 fused to mTurquoise2DepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 2
Plasmid#51761PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 2
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralExpressionMammalianPromoterIn frame with Cas9Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA-MS2x2-TagRFP657
Plasmid#184441PurposesgRNA with MS2 aptamer, TagRFP657 as selection markerDepositorInsertsgRNA-MS2x2, TagRFP657
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Syntaxin1A
Plasmid#170871PurposeMammalian expression of His-GFP-Syntaxin1A.DepositorAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205318PurposeAll-in-one AAV vector expressing ISDge10 TnpB and its reRNADepositorInserthumanized ISDge10 TnpB
UseAAVTags3 x HAExpressionMammalianAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL
Plasmid#208849PurposeExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL
Plasmid#208850PurposeExpresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
shTCF7L2 # 1
Plasmid#42557DepositorAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VP192-T2A-EGFP-shP53
Plasmid#69535PurposeEpisomal plasmid encoding dCas9VP192 and p53 shRNADepositorInsertdCas9-dCas9VP192-GFP-shp53
UseCRISPRTagsEGFPPromoterCAGAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
LEGO-AP1x6-GM55
Plasmid#217415PurposeLuciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseExpressionMammalianPromoter6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GUCY2C
Plasmid#116749PurposeLentiviral expression of GUCY2CDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGES201
Plasmid#190197PurposeFor CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Plasmid#98928PurposeCre dependent AAV expression of Ruby2 spaghetti monster FLAG constructDepositorHas ServiceAAV1 and AAV5InsertRuby2-smFP-Flag
UseAAVExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191537PurposeOverexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-ddMisCas13b-SNAPf-NLS-3xFlag
ExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-VP64-dCas9-VP64
Plasmid#232179PurposeLentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cellsDepositorInsertVP64-dCas9-VP64
UseLentiviralExpressionMammalianMutationD10A, H840APromoterhUbCAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pCR4-24XPP7SL
Plasmid#31864DepositorInsert24xPP7 Stem Loop Cassette
Available SinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-mScarlet-I
Plasmid#182296PurposeFluorescent reporter for AhR activityDepositorInsertmScarlet-I
Tagsm-Scarlet-IExpressionMammalianAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP4-8
Plasmid#79813PurposeModified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugationDepositorInsertGentamicin resistance gene
UseHelper bhr plasmid for mobilizing other plasmids …PromoterOwn promoterAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-C1-FKBP-PIP5K1C-HD-EE
Plasmid#220090PurposeRecruitable PIP5K to make PIP2DepositorInsertPIP5K1C (PIP5K1C Human)
TagsTagBFP-FKBP1A(3-108)ExpressionMammalianMutationamino acids 1-635; D101R, R304D, R445E, K446EPromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-INHBA
Plasmid#73015PurposeRetroviral expression of human activin ADepositorAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-229E-Nucleocapsid
Plasmid#168967PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-Nucleocapsid (N )
UseGateway-compatible entry vectorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-Nucleocapsid
Plasmid#168947PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-Nucleocapsid (EYW02_gp8 )
UseGateway-compatible entry vectorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorInsertTn5
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-1397C-AD
Plasmid#183892PurposeExpresses TALED_Left-ND1-1397C-AD in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only
