164,909 results

1. pEGFPC1-muscle Amphiphysin 2

   Plasmid

   #22213

   Depositor

   Pietro De Camilli

   Article

   Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

   Insert

   Amphiphysin II (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 35S-GWY-GAL4BD/pAM

   Plasmid

   #201231

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-GAL4BD
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-mCherry-FLAG-CD59-GPI

   Plasmid

   #50378

   Purpose

   Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Article

   Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   etfu and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV Luciferase PGK-hygro

   Plasmid

   #18782

   Depositor

   Scott Lowe

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Use

   Tags

   Palmitoylation signal*

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dyn2-pmCherryN1

   Plasmid

   #27689

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Dynamin 2 (DNM2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA

    Plasmid

    #163489

    Purpose

    Direct-expressing oNigri AAV Virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Has Service

    AAV1

    Insert

    oNigri
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin promoter

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK168_mCherry-H2B

    Plasmid

    #46363

    Purpose

    visualizes chromosomes

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D26G, V119I and S125D

    Promoter

    pBABE

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-GFP [N86/8R]

    Plasmid

    #177572

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GLP1R-Tango

    Plasmid

    #66295

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTetOne-mCherry-cNLS

    Plasmid

    #138527

    Purpose

    Inducible expression of mCherry with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Use

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Perox-RA

    Plasmid

    #209869

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p6XHis_NLS-SaCas9

    Plasmid

    #101086

    Purpose

    Express SaCas9 in bacteria with a 6xHis tag for purification

    Depositor

    Rick Tarleton

    Article

    Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

    Insert

    CRISPR-associated protein Cas9/Csn1
    

    Use

    CRISPR

    Tags

    TrxA-6xHis-STag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229785

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CAG-GFP (AAV2 trial size)

    Viral Prep

    #37825-AAV2.T

    Purpose

    Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

26. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TB201

    Bacterial Strain

    #230031

    Purpose

    Fluorescently labelled (YFP) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

28. EGFP-Rab11a-7

    Plasmid

    #56444

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. EGFP-Tau(T217A)

    Plasmid

    #187025

    Purpose

    Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 217 to Alanine

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV hSyn-fDIO-taCasp3-TEVp

    Plasmid

    #207997

    Purpose

    Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

    Depositor

    Kazunari Miyamichi

    Article

    Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

    Insert

    taCasp3-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTOX5

    Plasmid

    #127450

    Purpose

    Encodes MqsR toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    mqsR toxin
    amilCP chromoprotein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag-ZDHHC17

    Plasmid

    #231665

    Purpose

    Expresses human ZDHHC17protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC17 (ZDHHC17 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Syn-Chronos-GFP (AAV1)

    Viral Prep

    #59170-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Syn-driven Chronos-GFP expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    GFP

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

36. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Use

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. dCas12Max-ABE

    Plasmid

    #195335

    Purpose

    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-ABE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-N1-Tspan4

    Plasmid

    #210323

    Purpose

    Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

    Depositor

    Li Yu

    Article

    Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

    Insert

    Tetraspanin 4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge