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Showing: 10401 - 10440 of 160936 results

  14. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  15. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. pMpGWB120

    Plasmid
    #68574
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68574-plasmid-map-sequence-id-121818

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  19. pcDNA3-His:CRY2:nls:VPR

    Plasmid
    #135989
    Purpose
    Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
    Depositor
    Roman Jerala
    Article
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Inserts
    CRY2 domain fused to the VPR activation domain
    CRY2 domain fused to the VPR activation domain
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135989-plasmid-map-sequence-id-269558

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  21. pSBtet-BH

    Plasmid
    #60499
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60499-plasmid-map-sequence-id-97548

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  25. AICSDP-58: NUP153-mEGFP

    Plasmid
    #114407
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153
    Depositor
    Allen Institute for Cell Science
    Insert
    NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114407-plasmid-map-sequence-id-220837

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  26. LV-AR

    Plasmid
    #213819
    Purpose
    Lenti-viral expression of human androgen receptor wild-type (WT).
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR (human androgen receptor) (AR Human)
    Use
    Lentiviral
    Tags
    Flag (N terminal on backbone)
    Expression
    Mammalian
    Mutation
    No, it is AR wild-type.
    Promoter
    EF-1a
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213819-plasmid-map-sequence-id-422263

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  29. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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Showing: 10401 - 10440 of 160936 results