We narrowed to 8,446 results for: set

1. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212831

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA320

   Plasmid

   #215952

   Purpose

   Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA315

   Plasmid

   #215951

   Purpose

   Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA074

   Plasmid

   #215933

   Purpose

   Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   H1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA435

   Plasmid

   #215962

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA021

   Plasmid

   #215929

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AA083

   Plasmid

   #215934

   Purpose

   Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-PARP1–eGFP

   Plasmid

   #211552

   Purpose

   A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-V5-LMNB1

   Plasmid

   #207779

   Purpose

   Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. qTAG-C-mNeon-Blast-H3C2

    Plasmid

    #207782

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_068

    Plasmid

    #164555

    Purpose

    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid

    #207766

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro

    Plasmid

    #202800

    Purpose

    Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFalpha (with intron)

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GG-EBNA-MIR302-7guides-PGK-Puro

    Plasmid

    #201960

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-7g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgPrdm9#1/Cre

    Plasmid

    #193231

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgPrdm9#1 (Prdm9 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-sgPrdm9#2/Cre

    Plasmid

    #193232

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgPrdm9#2 (Prdm9 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pv9_MBD1_MBD-R22A-13XL-BASU

    Plasmid

    #179417

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-R22A-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid

    #179402

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Phd domain DW890/891AA

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_AU1.C.NWS_mCherry-NLS

    Plasmid

    #178282

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.NWS and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid

    #179893

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid

    #179895

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO_NWS.S.V5_mCherry-NLS

    Plasmid

    #178281

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.S.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO_NWS.Ollas.V5_mCherry-NLS

    Plasmid

    #178280

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.Ollas.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO_HSV.NWS.V5_mCherry-NLS

    Plasmid

    #178275

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO_HSV.NWS.VSVg_mCherry-NLS

    Plasmid

    #178274

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO_HSV.NWS.Ollas_mCherry-NLS

    Plasmid

    #178272

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.Ollas and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO_HA.VSVg.V5_mCherry-NLS

    Plasmid

    #178271

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO_HA.S.V5_mCherry-NLS

    Plasmid

    #178270

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.S.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO_HA.Ollas.V5_mCherry-NLS

    Plasmid

    #178268

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.Ollas.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO_HA.Ollas.VSVg_mCherry-NLS

    Plasmid

    #178267

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.Ollas.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge