We narrowed to 5,725 results for: chia

1. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas-CmR(+)

   Plasmid

   #113633

   Purpose

   Chloramphenicol resistant derivative of pCas plasmid

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   Chloramphenicol resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Pcat

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV2_CAG_oROS-G

   Plasmid

   #216115

   Purpose

   Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBLADE(FP6*)-mCherry

   Plasmid

   #168048

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Inserts

   Blue light-inducible AraC dimers in Escherichia coli
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101* and PBAD

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM627

   Plasmid

   #112532

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107, PL(HrtO), and ProD

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCD12

   Plasmid

   #225541

   Purpose

   Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-BirA-P2A-mCherry

   Plasmid

   #74056

   Purpose

   retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   BirA biotin ligase
   

   Use

   Retroviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTRs

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2362_Tier3(SB)-rtTA-PuroR

   Plasmid

   #169658

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKD068

    Plasmid

    #136471

    Purpose

    SSB-GFP IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-GFP (ssb E. coli)
    

    Use

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC3.1_CMV_oROS-G

    Plasmid

    #216111

    Purpose

    Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLS.419

    Plasmid

    #187208

    Purpose

    Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco4 ncRNA
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid

    #174692

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dead Streptavidin-SpyCatcher

    Plasmid

    #59547

    Purpose

    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead-dloop-Streptavidin-SpyCatcher
    

    Use

    Tags

    N-terminally shortened SpyCatcher

    Expression

    Bacterial

    Mutation

    PolyAsp in L3/4 loop for ion-exchange

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

    Plasmid

    #176587

    Purpose

    Bacterial expression of His14-MBP-TEV-AmaTnpB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Use

    Tags

    His14 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNBU2_erm_SIE1

    Plasmid

    #136356

    Purpose

    Selection by Inducible Effectors Test Plasmid

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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