We narrowed to 18,492 results for: REV

1. pPROEX Hsp104 (1-575)

   Plasmid

   #1318

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 (1-575) (HSP104 Budding Yeast)
   

   Tags

   6X His + TEV cleavage

   Expression

   Bacterial

   Mutation

   aa1-575 of Hsp104

   Available Since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-WIPI2 HRD

   Plasmid

   #207544

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human WIPI2 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH5

   Plasmid

   #52531

   Purpose

   expresses FLP recombinase in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   FLP recombinase (flp Budding Yeast)
   

   Expression

   Insect

   Promoter

   ubiquitin

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415TEF cyto roGFP2-Grx1(yeast)

   Plasmid

   #65004

   Purpose

   Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   Glutaredoxin-1
   

   Tags

   roGFP2

   Expression

   Yeast

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ABKAR(T/A)

   Plasmid

   #140052

   Purpose

   Negative control mutant for ABKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-QFGal4-SV40pA

   Plasmid

   #155126

   Purpose

   In vitro transcription of QFGal4 mRNA

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4
   

   Use

   Expression in zebrafish

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ptRNA100

    Plasmid

    #154133

    Purpose

    Expresses a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    tRNAs operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBacT7-S1T3D

    Plasmid

    #33282

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

    Insert

    S1 (S1 Orthoreovirus T3D)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMV-VioD

    Plasmid

    #65350

    Purpose

    systhesis of vioD gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioD
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. tCRMod-GGSGGS9

    Plasmid

    #111762

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS9-mRuby2 fusion
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Puro-iDEST

    Plasmid

    #75336

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXD70LacZ2-Pdadh-LacZ

    Plasmid

    #191618

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-NEAT1m_v1

    Plasmid

    #97083

    Purpose

    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1m_v1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Tags

    3xFLAG

    Expression

    Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. Marathon-RT tetramutant for Split-PE (pEH101)

    Plasmid

    #190109

    Purpose

    Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, D74R, N116K, N197R

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pKMV-VioB

    Plasmid

    #65348

    Purpose

    systhesis of vioB gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioB
    

    Use

    Synthetic Biology

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pPpT4- PGK1prom-TIR1-FLAG

    Plasmid

    #189724

    Purpose

    Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Expression

    Yeast

    Promoter

    PGK1 promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pX335-NEAT1_IS_v1

    Plasmid

    #97085

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRK-flag-USP19 C506S

    Plasmid

    #78584

    Purpose

    Expression flag-USP19 C506S in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 C506S (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 506 cysteine residue to serine

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

29. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Promoter

    EFs

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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