179,955 results
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Bacterial strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial resistanceKanamycinAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pSTARR-seq_human
Plasmid#71509PurposehumanSTARR-seq screening vector with SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionMammalianAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Strain SIJ_488
Bacterial strain#68246PurposeE. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.DepositorBacterial resistanceNoneAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Mem
Plasmid#55779PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Mem
TagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianPromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-AtU6-29
Plasmid#66203Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-AtU3b
Plasmid#66198Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterAtU3bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC13N-iCAG.copGFP
Plasmid#66578PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENsDepositorInsertcopGFP
ExpressionMammalianPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
single polypeptide FPX biosensor for caspase-3
Plasmid#60883PurposeFPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsingle polypeptide FPX biosensor for caspase-3
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1285
Plasmid#61252PurposeCRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
UseCRISPRExpressionWormAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mOrange-N1
Plasmid#54499PurposeLocalization: N1 Cloning Vector, Excitation: 548, Emission: 562DepositorTypeEmpty backboneTagsmOrangeExpressionMammalianAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50454PurposeDouble floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorHas serviceAAV8InserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II
Plasmid#52092PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with EGFPDepositorInsertsUseRetroviralTagsF2A, IRES-EGFP, P2A, and T2AExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-dV-IgG1/λ
Plasmid#52214PurposeHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin lambda constant region
ExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-myc-Miro2
Plasmid#47891PurposeExpresses myc tagged Miro2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiro2 (RHOT2 Human)
TagsmycExpressionMammalianPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.20-HIF1A prom
Plasmid#40173DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIF1A promoter fragment (HIF1A Human)
ExpressionMammalianAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LYN11-FRB-mcherry
Plasmid#38004DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmCherryExpressionMammalianAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGCDNsam-Hnf4a-IRES-GFP
Plasmid#33006DepositorInserthepatic nuclear factor 4 alpha (Hnf4a Mouse)
UseRetroviralAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HA-MST2
Plasmid#33098DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMST2 (STK3 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-K-RAS V12
Plasmid#31200PurposeGateway donor vector containing KRAS G12V.DepositorAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSVL(D3)
Plasmid#29335DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrimer of the HDV genome
ExpressionMammalianAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by