Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 34,066 results for: crispr

Clear keywords
Showing: 5281 - 5300 of 34066 results

  2. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47912-plasmid-map-sequence-id-68534

    Enlarge

  4. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68416-plasmid-map-sequence-id-120194

    Enlarge

  8. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

    Enlarge

  9. Lenti-LbCpf1-Blast

    Plasmid
    #84751
    Purpose
    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    LbCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84751-plasmid-map-sequence-id-164465

    Enlarge

  10. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

    Enlarge

  12. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60551-plasmid-map-sequence-id-97050

    Enlarge

  15. pJHG003

    Plasmid
    #126454
    Purpose
    Expresses Cas9-XTEN-dTdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    Added D343E and D345E to inactivate TdT.
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126454-plasmid-map-sequence-id-245973

    Enlarge

Showing: 5281 - 5300 of 34066 results