We narrowed to 92,342 results for: lias
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Plasmid#24887DepositorInserthypoxia inducible factor1alpha (HIF1A Human)
TagsGal4dbd and HA-tagExpressionMammalianMutationpartial HIF1alpha cDNA (encoding aa 530-652)fused…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c-AtMIR173
Plasmid#137885PurposePlant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.DepositorInsertsAtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos
ExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Available SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag-SKP1-T131A
Plasmid#213734PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q
Plasmid#60420PurposeMammalian Expression of ITSN DH/PH-tgRFPt-MicroDepositorInserthITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationhITSN 1159-1509 // SSPB R73Q, Y11K and A15EPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-mBVRA
Plasmid#100286PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for mouse BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TNT-CFP-STIM1-C227W
Plasmid#68403PurposeExpresses a STIM1 gain-of-function mutant in mammalian cellsDepositorInsertCFP-STIM1 (STIM1 Human)
TagsCyan Fluorescent Protein (CFP)ExpressionMammalianMutationChanged Cysteine 227 to Tryptophan; CFP was inser…PromoterCMVAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-E138A
Plasmid#69921PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationE138APromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC
Plasmid#69587PurposeExpresses mutated p53-L344P tagged with mKate2 and split C-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-G117S
Plasmid#69920PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationG117SPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-DipA-ss
Plasmid#137068PurposeE. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature DipA: lacks the DipA signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-D176H
Plasmid#69919PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationD176HPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
TagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
Plasmid#69586PurposeExpresses mutated p53-L344P tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387E
Plasmid#59906PurposeExpresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationS387E, phospho-dominantPromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Neo-2/15-TGFa-SPMn-SpyTag003
Plasmid#246650PurposeExpresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)DepositorInsertNeo-2/15-TGFa-SPMn-SpyTag003
TagsSignal peptide and SpyTag003ExpressionMammalianMutationConstruct contains the tPA signal peptide, Neo2/…PromoterCMV promoterAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-HA-Gnas
Plasmid#245994PurposeExpress HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only