We narrowed to 21,829 results for: SPR

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pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#195542

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170995

Purpose

HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-UPEmS-tevo

Plasmid

#228894

Purpose

Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pegRNA2_tevopreQ1

Plasmid

#212039

Purpose

Bacterial expression of the pegRNA for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

pegRNA

Use

CRISPR

Promoter

J23119

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Expression

Bacterial

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ142-ZmUbi

Plasmid

#138106

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-pT

Plasmid

#138108

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pMOD_A0508

Plasmid

#91014

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pV1081

Plasmid

#111425

Purpose

Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgADE-NT2

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-NOR

Plasmid

#62306

Purpose

synthetic circuit: NOR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PPhlF and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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