We narrowed to 4,125 results for: p2a
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Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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TRE3G-PE2-P2A-BFP
Plasmid#231580PurposeExpresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA proteinDepositorInsertPE2-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode
Plasmid#170237PurposeBarcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-p300-P2A-CASANOVA
Plasmid#113129PurposeCo-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVADepositorInsertdCas9-p300-P2A-CASANOVA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA
Plasmid#230988PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized optopatch for neuronal dendrites
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-bReaches-TS
Plasmid#158701PurposeReporter constructDepositorInsertmCherry-p2A-bReaches-TS
UseAAVTagsmCherryExpressionMammalianMutationPromoterTREAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV40-mCherry-P2A-Hygro
Plasmid#106478PurposeExpression of mCherry and Hygromycin resistance gene from SV40 promoterDepositorInsertsmCherry
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK344 (Hygro-P2A-mAID)
Plasmid#121178PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII
Plasmid#165431PurposeExpresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.DepositorInserttdTomato-P2A-paCaMKII
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationC124SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-Magneto2.0-p2A-mCherry
Plasmid#74308PurposeBicistronic expression of Magneto2.0 and mCherry in mammalian cellsDepositorUseTagsFlag and p2A-mCherryExpressionMammalianMutationTRPV4: delta 760–871PromoterCMVAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiCROP-PuroR-P2A-GFP-U6
Plasmid#200029PurposeLentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseLentiviralTagsPuroR-P2A-GFPExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.10_Gag-MCP-P2A-eGFP
Plasmid#205525PurposeExpress HIV Gag-MCP-P2A-eGFPDepositorInsertGag-MCP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-Chrimson-TS
Plasmid#158702PurposeReporter constructDepositorInsertTRE-mcherry-p2A-Chrimson-TS
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTREAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only