We narrowed to 16,596 results for: puromycin

1. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175508

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFH2.155_LV-Puro-T2A-mTagBFP2

   Plasmid

   #205553

   Purpose

   Express puromycin resistance marker and mTagBFP2

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Puro-T2A-mTagBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMaCTag-P29

   Plasmid

   #120040

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCuo DN Rac1

   Plasmid

   #84644

   Purpose

   dominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Promoter

   cumate-inducible promoter

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

6. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV/U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

   Plasmid

   #175509

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRDA_888

   Plasmid

   #201165

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHLuc (Antares-SEPLuc C1A4E+6)

   Plasmid

   #141089

   Purpose

   final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pscALPS- HIV2-ROD vpx

    Plasmid

    #115816

    Purpose

    Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV2ROD Vpx (M15390)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

    Plasmid

    #187925

    Purpose

    sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

    Depositor

    Martin Turner

    Article

    Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

    Insert

    hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSUPER-retro-puro-tetO-CTR1-shRNA

    Plasmid

    #53180

    Purpose

    Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAIP hGMCSF co

    Plasmid

    #74168

    Purpose

    Lentiviral vector for expression of human codon optimized GMCSF with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GMCSF (CSF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CMV-cGASΔN-HA

    Plasmid

    #130912

    Purpose

    Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid

    #175507

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRRL-gRNA(anti-cGAS)-Cas9-Puro

    Plasmid

    #130908

    Purpose

    gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P26

    Plasmid

    #120037

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Promoter

    TRE

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pscALPS-SIV-CPZ-TAN3 vpr

    Plasmid

    #115833

    Purpose

    Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVCPZ-TAN3 Vpr (DQ374658)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge