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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag9

Plasmid

#175545

Purpose

Expression of HaloTag9 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ1658-dCas9-EGFP

Plasmid

#51023

Purpose

Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

dCas9 fuse to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GBX

Plasmid

#64123

Purpose

a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

GFP-2A-BCL2L1 (BCL2 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Article

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pT3-EF1α-Dest

Plasmid

#133299

Purpose

The pT3-EF1α destination vector without any insert which can be used for LR reaction.

Depositor

Xin Chen

Article

Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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JEC1576

Plasmid

#253203

Purpose

pSynORI (Cumate-inducible)

Depositor

James Chappell

Article

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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JEC1575

Plasmid

#253202

Purpose

pSynORI (IPTG-inducible)

Depositor

James Chappell

Article

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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JEC1569

Plasmid

#253201

Purpose

pSynORI

Depositor

James Chappell

Article

Liu et al Nat Commun. 2026 Feb 2. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Zeo (637-7)

Plasmid

#17449

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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Human SAM library in lenti sgRNA(MS2)_puro backbone

Pooled Library

#1000000074

Purpose

Genome-wide CRISPR gRNA pooled library for activation of mouse genes

Depositor

Feng Zhang

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

Plasmid

#213027

Purpose

Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag

Tags

3xFlag (ORF2p)

Expression

Mammalian

Mutation

D702Y (RT- reverse transcriptase catalytic dead)

Promoter

CMV

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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