164,852 results

1. PT3.5/CMV-EGFRvIII

   Plasmid

   #20280

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   EGFRvII (EGFR Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   See depositor comments below.

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPW3758 : CMVd3-HsIRE1-HaloTag

   Plasmid

   #185680

   Purpose

   Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NeonGreen-C1-ORP5

   Plasmid

   #220071

   Purpose

   overexpresses ORP5 with a neonGreen fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5 (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

   Plasmid

   #197510

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Expression

   Mutation

   ABE8e mutations in TadA and nSpRY(D10A/A61R)

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TC358

   Plasmid

   #164885

   Purpose

   TRE::GFP, inducible GFP expression when co-express with rtTA

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-HA [12CA5] - Chimeric (Recombinant Antibody)

   Antibody

   #199805-rAb

   Purpose

   Anti-HA chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.

   Depositor

   Melina Fan

   Recommended Applications

   Western Blot

   Reactivity

   Source Species

   Chicken

   Isotype

   IgY

   Trial Size

   Available to purchase

   Available since

   April 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

7. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_nV5-AMOTL1

   Plasmid

   #172998

   Purpose

   constitutive expression of V5-tagged AMOTL1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL1 (AMOTL1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-Ii_SBP-mCherry-Golgin84

    Plasmid

    #65304

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Ub KO.G76V

    Plasmid

    #11932

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin KO.G76V (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Conjugation deficient mutant. All 7 lysines of ub…

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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