318,318 results

1. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Promoter

   human PGK promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Promoter

   human PGK promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Promoter

   CMV immearly promotor

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Promoter

   CMV immearly promotor

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERK-KTR-mStrawberry reporter

   Plasmid

   #158686

   Purpose

   This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ERK-KTR-mStrawberry reporter

   Plasmid

   #158686

   Purpose

   This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1A-CD19.28H.28TM.28.3z

   Plasmid

   #200678

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1A-CD19.28H.28TM.28.3z

   Plasmid

   #200678

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PJEBAN3

    Plasmid

    #203682

    Purpose

    Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    Yellow Fluorescent Protein (YFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PJEBAN3

    Plasmid

    #203682

    Purpose

    Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    Yellow Fluorescent Protein (YFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Marionette Sensor Collection

    Plasmid Kit

    #1000000137

    Purpose

    The Marionette Sensor Collection enables the modular control of up to 12 genes, by placing each one under the control of a small inducible promoter.

    Depositor

    Christopher Voigt

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP

    Plasmid

    #85426

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-2
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP

    Plasmid

    #85426

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-2
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoter

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoter

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mRuby2-N1

    Plasmid

    #54614

    Purpose

    Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. mRuby2-N1

    Plasmid

    #54614

    Purpose

    Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral Prep

    #162378-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

25. pLenti6/V5-p53_R175H

    Plasmid

    #22936

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R175H Mutant

    Available Since

    March 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti6/V5-p53_R175H

    Plasmid

    #22936

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R175H Mutant

    Available Since

    March 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV1)

    Viral Prep

    #130998-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    EF1a

    Tags

    mScarlet

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

30. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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