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169,450 results

Showing: 8101 - 8130 of 169450 results

  3. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  7. pAAV-CaMKIIa-mCherry (AAV Retrograde)

    Viral Prep
    #114469-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-490369

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  14. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Available Since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

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  21. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Promoter
    TEF1p
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

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  26. pRK5-myc-RhoA-wt

    Plasmid
    #12962
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 8101 - 8130 of 169450 results