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We narrowed to 45,509 results for: cha

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Showing: 10801 - 10840 of 45509 results

  7. B4GALT1-Dox

    Plasmid
    #124633
    Purpose
    Doxycycline-inducible B4GALT1 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    Expression
    Mammalian
    Promoter
    TRE-tight and hEF1a
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124633-plasmid-map-sequence-id-248076

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  8. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154066-plasmid-map-sequence-id-312954

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  11. p-mCherry-C1-iC++

    Plasmid
    #98165
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iC++ gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98165-plasmid-map-sequence-id-182714

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  23. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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  29. pMinDis.X513-iNicSnFR3b-(V7)-PM

    Plasmid
    #125124
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3b-(V7)-PM
    Tags
    Ig-K-Chain and membrane anchoring protein-PDGFR
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125124-plasmid-map-sequence-id-245438

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  30. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. IFITM3-N21del-V93-TAG

    Plasmid
    #122480
    Purpose
    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon and deleted amin…
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122480-plasmid-map-sequence-id-237764

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  37. p3E DN Kif5a

    Plasmid
    #105973
    Purpose
    3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    Kif5a (kif5aa Zebrafish)
    Expression
    Bacterial
    Mutation
    truncation of aa 416-1033
    Available Since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105973-plasmid-map-sequence-id-203068

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  38. pFUSEss-CHIg-mG3_Lys322Arg

    Plasmid
    #105862
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    Lys322Arg in constant region of IgG1 heavy chain
    Promoter
    hEF1-HTLV prom
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105862-plasmid-map-sequence-id-206034

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  39. pAM4663

    Plasmid
    #85126
    Purpose
    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.
    Depositor
    Susan Golden
    Article
    Chang et al Science. 2015 Jul 17;349(6245):324-8. do
    Insert
    PkaiB-luciferase
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85126-plasmid-map-sequence-id-180127

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Showing: 10801 - 10840 of 45509 results