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Plasmid#193279PurposeExpression of CBE-T1.28 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.28
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.30
Plasmid#193280PurposeExpression of CBE-T1.30 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.30
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO
Plasmid#192382PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.DepositorInsertAct2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3
Plasmid#192397PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).DepositorInsert35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)
Plasmid#191165PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTRDepositorInsertmCherry:s70587-3xMS2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA
Plasmid#191148PurposeAAV expression of a Cre reporter with EYFP output; derived from a gift from prof. Liqun LuoDepositorInsertDIO-EYFP
UseTagsExpressionMammalianMutationWTPromoterhEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc0
Plasmid#186771PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hNluc
Plasmid#186772PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::hNluc
Plasmid#186774PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc1
Plasmid#186754PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL10::sNluc1
Plasmid#186756PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc1
Plasmid#186757PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc0
Plasmid#186758PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc2
Plasmid#186759PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc3
Plasmid#186760PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc4
Plasmid#186761PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc5
Plasmid#186762PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc2_STOP
Plasmid#186767PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.DepositorInsertNanoluciferase
UseTagsExpressionMutationPremature stop codon at residue 16PromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo-W35A
Plasmid#179397PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)DepositorInsertCBX7_2xChromo(W35A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_3EGFP
Plasmid#185967PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPr-Egfp cassette in forward direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo-W42A
Plasmid#179394PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)DepositorInsertCBX1_2xChromo(W42A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W42APromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv7_MBD1_MBD_noEGFP
Plasmid#179406PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv7_CBX1_2xChromo_noEGFP
Plasmid#179395PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFPDepositorInsertCBX1_2xChromo_noEGFP
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD-R22A
Plasmid#179405PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)DepositorInsertMBD1_MBD(R22A)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R22APromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1010A
Plasmid#176078PurposepBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010B
Plasmid#176079PurposepBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010C
Plasmid#176080PurposepBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010D
Plasmid#176081PurposepBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC51
Plasmid#104825PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoterDepositorInsertGlyma.12g075700, Glyma.11g145900
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only