We narrowed to 9,180 results for: mel

1. pAFMW-Nbr[C399-625]-Gwpep(Mm)

   Plasmid

   #188589

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and GWpep mm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAFMW-Nbr[C399-625]-Gwpep(Dm)

   Plasmid

   #188590

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAFMW-Gwpep(Dm)-Nbr[C399-625]

   Plasmid

   #188592

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAFMW-Gwpep(Mm)-Nbr[C399-625]

   Plasmid

   #188591

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep mm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNHT7

   Plasmid

   #186753

   Purpose

   A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Type

   Empty backbone

   Use

   Synthetic Biology; Cloning backbone

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-sgRNA-Cas9.dora_1

   Plasmid

   #190700

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 1
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc-sgRNA-Cas9.dora_2

   Plasmid

   #190701

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-sgRNA-Cas9.dora_3

   Plasmid

   #190702

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 3
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv6_dPC_2xChromo

   Plasmid

   #179398

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1056H

    Plasmid

    #183135

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1056J

    Plasmid

    #183136

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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