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MINK1 gRNA (BRDN0001147795)

Plasmid

#76262

Purpose

3rd generation lentiviral gRNA plasmid targeting human MINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MINK1 (MINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
DGUOK gRNA (BRDN0001147023)

Plasmid

#76602

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGUOK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGUOK (DGUOK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001148670)

Plasmid

#77564

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001146365)

Plasmid

#77565

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001144918)

Plasmid

#77566

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Stx3 triple mutant (F31A D33A E34A)

Plasmid

#86945

Purpose

Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

Depositor

Thomas Weimbs

Article

Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

Insert

STX3 triple mutant (F31A D33A E34A) (STX3 Human)

Tags

myc-His6

Expression

Mammalian

Mutation

triple mutant (F31A D33A E34A)

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(100-263)

Plasmid

#72548

Purpose

expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3xFlag and SV40 NLS

Expression

Mammalian

Mutation

100-263 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A-Blast

Plasmid

#248032

Purpose

Constitutively expresses myc tagged membrane bound IL15, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL15 (IL15 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Human, Mustard Weed)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-IRES2-CRY2-STIM1(238-448)

Plasmid

#248295

Purpose

An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP and CRY2-fused STIM1 fragment (a.a.238-448) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-237,449-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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SFFV-NR3C1-Brd 106

Plasmid

#219076

Purpose

Transcription factor NR3C1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

NR3C1 (NR3C1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Egr1-CytoTape-V5

Plasmid

#239428

Purpose

CytoTape signal monomer for recording Egr1 promoter transcriptional activity

Depositor

Changyang Linghu

Article

Zheng et al bioRxiv [Preprint]. 2025 May 10:2025.05.

Insert

CytoTape-V5 (EGR1 Synthetic)

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

Egr1 promoter

Available Since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnas

Plasmid

#245994

Purpose

Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnas (Gnas Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreen

Plasmid

#244172

Purpose

Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (75S) (KDR Human, Synthetic)

Use

Synthetic Biology

Tags

VEGFR2 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_4

Plasmid

#242899

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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