We narrowed to 2,423 results for: LRE

1. pAAV-FLEX-SaCas9-U6-sgSlc17a8

   Plasmid

   #124848

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-TCAB1 HRD

   Plasmid

   #207535

   Purpose

   Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK288 (mAID-Bsr)

   Plasmid

   #72826

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Bsr
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

   Plasmid

   #62956

   Purpose

   Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAT005

   Plasmid

   #171632

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFudio-Cpg15-IRES2-TdTomatoW

   Plasmid

   #134309

   Purpose

   Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

   Depositor

   Elly Nedivi

   Article

   Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

   Inserts

   cpg15 (Nrn1 Rat, Mouse)
   TdTomato
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

    Plasmid

    #62951

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

    Plasmid

    #62955

    Purpose

    Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. RT3GEPIR

    Plasmid

    #111169

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Donor-PIGA

    Plasmid

    #153530

    Purpose

    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    No mutation

    Promoter

    No promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-Limk1

    Plasmid

    #163414

    Purpose

    Expresses LIMK1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Limk1 (Limk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ER-ABKAR

    Plasmid

    #61508

    Purpose

    Expression of AMPK biosensor targeted to the endoplasmic reticulum

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    ER targeting sequence from CYP450 2C1 (see commen…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pSCMV-H2B-FT-Medium

    Plasmid

    #157670

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Medium-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

    Plasmid

    #62905

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP_mEGFP-GluA1c

    Plasmid

    #114136

    Purpose

    Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mEGFP tagged GluA1 C-terminus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

    Plasmid

    #62908

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-Bmp4

    Plasmid

    #163588

    Purpose

    Expresses BMP-4 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp4 (Bmp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

    Plasmid

    #62911

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Use

    Tags

    3x-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

    Plasmid

    #62914

    Purpose

    Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-Bmpr2

    Plasmid

    #163403

    Purpose

    Expresses BMPR-2 in mammalican cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge