We narrowed to 19,962 results for: omp

1. Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

   Pooled Library

   #136479

   Purpose

   Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

   Depositor

   Jonathan Weissman

   Article

   Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

2. UbiGate Components (set1)

   Plasmid Kit

   #1000000144

   Purpose

   Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays

   Depositor

   Marco Trujillo

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

3. Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

   Plasmid Kit

   #1000000087

   Purpose

   Tol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disorders

   Depositor

   Nicholas Cole

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

4. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

   Plasmid Kit

   #1000000221

   Purpose

   A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

   Depositor

   Eric Cantor

   Available since

   June 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

5. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Johannes Stuttmann, Sylvestre Marillonnet

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

6. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

7. C321.∆A ompT- rne- endA-

   Bacterial Strain

   #182774

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Ampicillin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

8. C321.∆A ompT- rne- lon-

   Bacterial Strain

   #182773

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Ampicillin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

9. C321.∆A T7RNAP ompT- rne- lon-

   Bacterial Strain

   #182778

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

10. C321.∆A ompT- rne- endA- lon-

    Bacterial Strain

    #182775

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pAL950

    Plasmid

    #119748

    Purpose

    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Internal NdeI site removed and mutations Alanine …

    Promoter

    T7

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSR62

    Plasmid

    #125076

    Purpose

    Expression of ompR under PLtetO-1, output promoter PompF146

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR (ompR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQZ083

    Plasmid

    #106417

    Purpose

    Express Complexin-1 fragment (1-83) in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Insert

    complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Fragment 1-83, Codon optimization

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-GFP-URA3-GFP

    Plasmid

    #72606

    Purpose

    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

    Depositor

    Bruce Granger

    Article

    Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

    Inserts

    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    

    Use

    Tags

    Expression

    Mutation

    Includes full 819 bp coding sequence of URA3, 435…

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-mCherry-Nanobody

    Plasmid

    #70696

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    mCherry-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV Flag WT-PTEN

    Plasmid

    #22231

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSR26-2

    Plasmid

    #104335

    Purpose

    Expresses ompR from a TetR reuglated promoter under inducible ATC control.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-IFT22

    Plasmid

    #218720

    Purpose

    Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT22 (RABL5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-mTcte1-PA

    Plasmid

    #176469

    Purpose

    Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    t-complex-associated testis expressed 1 (Tcte1 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N2-IFT20

    Plasmid

    #218719

    Purpose

    Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT20 (IFT20 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-C1-IFT38

    Plasmid

    #218723

    Purpose

    Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT38 (CLUAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-C1-IFT27

    Plasmid

    #218722

    Purpose

    Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT27 (IFT27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-EGFP-C-IFT46

    Plasmid

    #218724

    Purpose

    Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT46 (IFT46 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1-IFT25

    Plasmid

    #218721

    Purpose

    Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT25 (HSPB11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV Flag dPDZ-PTEN (1-400)

    Plasmid

    #22232

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (Pten Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    PDZ binding domain deleted. Contains amino acids …

    Promoter

    Available since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

28. C1QBP-bio-His

    Plasmid

    #53330

    Purpose

    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    C1QBP (C1QBP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-mDrc3-PA

    Plasmid

    #176467

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 3 (Drc3 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge