We narrowed to 16,183 results for: GRN

1. pU3-gRNA

   Plasmid

   #53063

   Purpose

   Transcription of guide RNA (gRNA) in rice cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   OsU3 promoter + gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Promoter

   rice U3

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMART-sgRNA (Sp)

   Plasmid

   #80427

   Purpose

   U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH-stsgRNA3.1

   Plasmid

   #64118

   Purpose

   Vector for expression of St1 sgRNA3.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA3.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAKN2_J23119-sgRNA

   Plasmid

   #108552

   Purpose

   Bacterial sgRNA expression vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   2x Streptococcus pyogenes sgRNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYLsgRNA-OsU6c

   Plasmid

   #66197

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU6c

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDKN1A sgRNA

   Plasmid

   #138189

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDKN1A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGmCherry-gRNA

   Plasmid

   #87110

   Purpose

   gRNA cloning vector with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 (Arabidopis)

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. psgRNAv1-empty

    Plasmid

    #171611

    Purpose

    sgRNA_v1 plasmid for AsCas12f1 in bacteria

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1_sgRNA_1
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6_rd6_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232433

    Purpose

    pegRNA with optimized 3' modifications to correct the rd6 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-FRT

    Plasmid

    #183241

    Purpose

    anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

    Depositor

    Karin Schnetz

    Article

    Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

    Insert

    sgRNA-FRT
    

    Expression

    Bacterial

    Promoter

    P-tet

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYLsgRNA-OsU3m

    Plasmid

    #66193

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU3m (SpeI site destroyed)

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. HaloKu70 sgRNA

    Plasmid

    #207583

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    GAGCAGTAGCCAACATGTCA
    

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p426_Cas9_gRNA-CAN1y

    Plasmid

    #87391

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEX-A-U6-gRNA

    Plasmid

    #65626

    Purpose

    Empty vector for the expression U6 driven gRNA

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    U6-gRNA
    

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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