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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/FGFR2myc

Plasmid

#33248

Depositor

Claudio Basilico, Alka Mansukhani

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Tags

His and Myc

Expression

Mammalian

Mutation

KpnI site introduced at stop codon of FGFR2 to be…

Promoter

CMV

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
Thy1-Brainbow-1.0 L

Plasmid

#18725

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; EYFP ; mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMcl-1

Plasmid

#32980

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
mTagBFP2-ZO1-C-14

Plasmid

#55335

Purpose

Localization: Tight Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ZO1 (TJP1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE ZO1myc

Plasmid

#30318

Depositor

Alan Fanning

Article

Fanning et al Mol Biol Cell. 2007 Mar . 18(3):721-31.

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

myc

Expression

Mammalian

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pC3.1-caggs-Crimson-CAAX

Plasmid

#190041

Purpose

Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Depositor

Michael Lin

Article

Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

Insert

Crimson

Tags

CAAX

Expression

Mammalian

Promoter

caggs

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pY011 (pcDNA3.1-hLb2Cpf1)

Plasmid

#69983

Purpose

Expresses humanized Lb2Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb2Cpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD51 (Mief1 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1941

Plasmid

#16510

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 1941 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600u

Plasmid

#13572

Depositor

Wolf Frommer

Article

Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

Insert

FLIPglu F16A

Tags

ECFP and EYFP

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap1mut

Plasmid

#8337

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap1mut (BIRC2 Human)

Tags

myc

Expression

Mammalian

Mutation

H588A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PDZ1

Plasmid

#59739

Purpose

expresses PDZ1 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Sept. 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GGamma9-GFP2

Plasmid

#140991

Purpose

Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma9-GFP2 (GNGT2 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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