We narrowed to 11,723 results for: Vars

1. pHH0103_GRB2-1/2

   Plasmid

   #91480

   Purpose

   Protein expression and purification of human SH3 domain construct GRB2-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   GRB2-1/2 (GRB2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLuc-PM-Venus

   Plasmid

   #164785

   Purpose

   Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Gaussia princeps)
   

   Tags

   GLuc and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.GFAP.SF-iGluSnFR.A184S

   Plasmid

   #106192

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-SARS-CoV-2 Δ18 B.1.351v2

   Plasmid

   #169463

   Purpose

   Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_ITK-1/1

   Plasmid

   #91253

   Purpose

   Protein expression and purification of human SH3 domain construct ITK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITK-1/1 (ITK Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist-SARS-CoV-2 Δ18 B.1.1.7

   Plasmid

   #169459

   Purpose

   Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-WT1

   Plasmid

   #192906

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of WT1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   WT1 (WT1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-PPARG

   Plasmid

   #192912

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   PPARG (PPARG Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 mitoAURKA

    Plasmid

    #157750

    Purpose

    Expression of AURKA targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    Aurora A without the first 30AA + MTS of CoxVIII

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-GSAS

    Plasmid

    #164565

    Purpose

    SARS-CoV-2 Spike protein (S-GSAS variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MS2_adRNA-MCP_ADAR2 DD_NES

    Plasmid

    #124706

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD_NES (RAB7A Human)
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid

    #106204

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S + mRuby3 fusion

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MS2_adRNA-MCP_ADAR1 DD_NES

    Plasmid

    #124630

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR1 DD_NES (RAB7A Human)
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-RR-ABE8e-V106W

    Plasmid

    #193656

    Purpose

    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_BCAR1-1/1

    Plasmid

    #91418

    Purpose

    Protein expression and purification of human SH3 domain construct BCAR1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BCAR1-1/1 (BCAR1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERP2-NR1H2

    Plasmid

    #192919

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR1H2 (NR1H2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pHH0103_FYN-1/1

    Plasmid

    #91413

    Purpose

    Protein expression and purification of human SH3 domain construct FYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYN-1/1 (FYN Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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