We narrowed to 15,455 results for: Pol;

1. LRG2.1_Puro

   Plasmid

   #125594

   Purpose

   Lentiviral expression of sgRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-ZNF598-TEV-3xFLAG

   Plasmid

   #105690

   Purpose

   Expresses C terminally 3xFLAG tagged ZNF598 protein

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Insert

   ZNF598 (ZNF598 Human)
   

   Tags

   TEV-3xFLAG

   Expression

   Mammalian

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPRIME-CMV-LNGFR-recipient

   Plasmid

   #11660

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   LNGFR

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-UBF

   Plasmid

   #17656

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   UBF (UBTF Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. p426 103Q GAL

   Plasmid

   #1188

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. YFC43

   Plasmid

   #89488

   Purpose

   gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

   Depositor

   Alejandro Ferrando

   Article

   Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

   Type

   Empty backbone

   Tags

   YFP-C (aa 154-240)

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFN43

   Plasmid

   #89487

   Purpose

   gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

   Depositor

   Alejandro Ferrando

   Article

   Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

   Type

   Empty backbone

   Tags

   YFP-N (aa 1-154)

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His-SCov2-nsp7

   Plasmid

   #154757

   Purpose

   Expression of SARS-CoV-2 nsp7

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-His-SCov2-nsp8

    Plasmid

    #154758

    Purpose

    Expression of SARS-CoV-2 nsp8

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBP-J23100

    Plasmid

    #72963

    Purpose

    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23100 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1_SEPT2

    Plasmid

    #71547

    Purpose

    mammalian expression of Sept 2 with a GFP fusion

    Depositor

    Elias Spiliotis

    Article

    Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

    Insert

    Septin 2 (Septin2 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE30 PTBP1 isoform1

    Plasmid

    #108591

    Purpose

    Recombinant His tagged protein production

    Depositor

    Chris Smith

    Article

    Wollerton et al RNA. 2001 Jun;7(6):819-32.

    Insert

    PTBP1 isoform 1 (PTBP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    isoform 1 (isoform c)

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. L1_CsR-Ck1

    Plasmid

    #136124

    Purpose

    L1 in position 1 (next to LB in L2 construct), for CsR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:mALS_3t35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. L1_HyR-Ck1

    Plasmid

    #136125

    Purpose

    L1 in position 1 (next to LB in L2 construct), for HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:hptII_3t35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA)

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCk4_spacer

    Plasmid

    #136074

    Purpose

    Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Flag-HA-PARP11

    Plasmid

    #22556

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. Akaluc-N1

    Plasmid

    #120371

    Purpose

    Akaluc luciferase mammalian expression in -N1 backbone

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc (human optimized)
    

    Expression

    Mammalian

    Mutation

    Human codon optimized

    Promoter

    CMV

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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