175,318 results

1. TREER-Actin

   Plasmid

   #208330

   Purpose

   TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TREER-Actin
   

   Tags

   TREER

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOMM20-PuRRRE

   Plasmid

   #208329

   Purpose

   PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TOMM20-PuRRRE
   

   Tags

   PuRRRE

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJR017

   Plasmid

   #198835

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::TeTX-SL2-mKate::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-Spe4-Kozak-miRFP713-RfA

   Plasmid

   #186381

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDx_mCherry-SYFP2

   Plasmid

   #189759

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mCherry-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SFRS10

   Plasmid

   #156007

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SFRS10 (TRA2B Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. F40F8.11 RNAi

   Plasmid

   #186731

   Purpose

   Knockdown of F40F8.11 gene

   Depositor

   Seung-Jae Lee

   Insert

   F40F8.11 targeting RNA
   

   Use

   RNAi

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-Spe4-RfA-miRFP713-stop

   Plasmid

   #186391

   Purpose

   Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-SKII-3XHA-eDHFR-NAT

    Plasmid

    #188926

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-eDHFR-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-Halo,IRES-NGFR

    Plasmid

    #164520

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_2pabPAC(Y72L)

    Plasmid

    #158970

    Purpose

    A negative control for AAV-mediated 2P-activatable bPAC

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPACY72L
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

    Plasmid

    #120567

    Purpose

    Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

    Plasmid

    #120566

    Purpose

    Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIPZ shcGAS-Hsa

    Plasmid

    #128175

    Purpose

    Doxycyclin inducible shRNA knockdown of human cGAS gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shcGAS Mmu (CGAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSNAP-tag (m) Vector

    Plasmid

    #101135

    Purpose

    Expression of protein of interest with SNAP-Tag in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    SNAP-tag (SNAP26m)

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV2)

    Viral Prep

    #100836-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    CAG

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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