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pLKO-puro-tetON-shOGDH 2956

Plasmid

#110943

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-short_BrokenHeart (no BC)

Plasmid

#86950

Purpose

AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

Brokenheart construct

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hGTB

Plasmid

#26196

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

F2A has 2A cleavage site deleted, producing htTA-…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pET-28a RUVBL2 Full Length

Plasmid

#159142

Purpose

Expresssion and protein purification of full length RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

Plasmid

#238003

Purpose

Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
AtSKP1 (SKP1 Mustard Weed)
AtCUL1 (CUL1 Mustard Weed)

Tags

S and T7

Expression

Bacterial

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-AR-S256A

Plasmid

#171227

Purpose

Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

Depositor

Steven Balk

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

Insert

human androgen receptor Ser256Ala mutant (AR Human)

Expression

Bacterial and Mammalian

Mutation

AR-Ser256Ala (AR-S256A)

Promoter

CMV

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-AR-S308A

Plasmid

#171229

Purpose

Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

Depositor

Steven Balk

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

Insert

human androgen receptor Ser308Ala mutant (AR Human)

Expression

Bacterial and Mammalian

Mutation

AR-Ser308Ala (AR-S308A)

Promoter

CMV

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-AR-S90A

Plasmid

#171225

Purpose

Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

Depositor

Steven Balk

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

Insert

human androgen receptor Ser90Ala mutant (AR Human)

Expression

Bacterial and Mammalian

Mutation

AR-Ser90Ala (AR-S90A)

Promoter

CMV

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT WT

Plasmid

#154282

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag PGC1 T177A S538A

Plasmid

#18093

Depositor

Bruce Spiegelman

Article

Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

Insert

PGC-1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

T177A S538A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Article

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKalpha

Plasmid

#15469

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR

Plasmid

#173940

Purpose

Bacterial expression of PARP1 lacking WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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