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Showing: 8521 - 8550 of 161241 results

  1. pCMV-SUN2 mScarlet

    Plasmid
    #187685
    Purpose
    In vivo visualization of SUN2
    Depositor
    Mary Beckerle
    Insert
    SUN2 (Sun2 Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187685-plasmid-map-sequence-id-371432

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  3. Tet-pLKO-FOXM1-shRNA-#3

    Plasmid
    #89500
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    89500-plasmid-map-sequence-id-180325

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  5. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

    Viral Prep
    #171027-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Patricia Jensen
    Article
    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi
    Promoter
    EF1a
    Tags
    tdTomato
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217342-plasmid-map-sequence-id-429351

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  16. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  18. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

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  19. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (AAV1)

    Viral Prep
    #98927-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (#98927). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of GFPsm_myc spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:
    Promoter
    CAG
    Tags
    GFPsm-myc (spaghetti monster construct)
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep
    #105677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  22. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13X
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  24. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

    Viral Prep
    #176751-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Tags
    None
    Available since
    Jan. 25, 2024
    Availability
    Academic Institutions and Nonprofits only

  26. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  30. mApple-Integrin-Beta1-N-18

    Plasmid
    #54914
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta1 (ITGB1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 8521 - 8550 of 161241 results