167,318 results

1. pLV-mTurquoise2-IL-1beta-mNeonGreen

   Plasmid

   #166783

   Purpose

   FRET analysis (pro-IL-1beta cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-IL-1b-mNeongreen (IL1B Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROP-PP-FBFP

   Plasmid

   #122137

   Purpose

   FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   FBFP
   

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1-F-tractin-mCherry

   Plasmid

   #155218

   Purpose

   Monitor global F-actin distribution

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

   Plasmid

   #211942

   Purpose

   dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

   Depositor

   Evgeny Kvon

   Article

   Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   HSP68

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA-Gent-EM7-Erv1p-PDI

   Plasmid

   #202486

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
   

   Expression

   Bacterial

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HyPer7-MEM

   Plasmid

   #136465

   Purpose

   Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Farnselation tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCGN-MEF2B

   Plasmid

   #32959

   Purpose

   For mammalian expression of human MEF2B with an HA tag

   Depositor

   Ron Prywes

   Article

   Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

   Insert

   MEF2B (MEF2B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV Retrograde)

    Viral Prep

    #162377-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

12. Twinkle-APEX2-V5

    Plasmid

    #129705

    Purpose

    APEX2 fused to the mitochondrial nucleoid protein Twinkle

    Depositor

    Alice Ting

    Article

    Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

    Insert

    Twinkle-APEX2-V5 (TWNK, APEX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPP5371

    Plasmid

    #128732

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163055

    Purpose

    Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiGuide-hygro

    Plasmid

    #160090

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV2)

    Viral Prep

    #105547-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

18. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. JM83

    Bacterial Strain

    #50348

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Messing et al Recombinant DNA Technical Bulletin, 1979

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. (SIM)10

    Plasmid

    #126946

    Purpose

    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. CMV-OMM-jRCaMP1b

    Plasmid

    #127871

    Purpose

    Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. mCherry-CRY2clust

    Plasmid

    #105624

    Purpose

    Expresses improved CRY2PHR homo-oligomerization

    Depositor

    Won Do Heo

    Article

    Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

    Insert

    mCherry-CRY2PHR-9aa (CRY2 Mustard Weed, Human)
    

    Tags

    9 amino acids

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. 3XFLAG-VP64-SadCas9-NLS-VP64

    Plasmid

    #135338

    Purpose

    3xFLAG 2xVP64 SadCas9 for single guide cloning

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    SadCas9
    

    Tags

    3xFLAG-VP64-SV40 NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. moxCerulean3

    Plasmid

    #68066

    Purpose

    C-terminal fusion to moxCerulean3

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Tags

    moxCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. MYC_pLX307

    Plasmid

    #98363

    Purpose

    Lentiviral expression of MYC

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    Non tagged Avidin
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Promoter

    rat Tyrosine hydroxylase

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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